![]() When an operation on a group is initiated, FlowJo can perform the same operation on every sample belonging to that group. Within a workspace, samples can be grouped or sorted by various attributes such as the panel of antibodies with which they are stained, tissue type, or patient from whom they came. FlowJo's ability to automate repetitive operations facilitates the production of statistics tables and graphical reports when the experiment involves many samples, parameters and/or operations. Viewing an entire experiment in a Workspace permits organizing and managing complex cytometry experiments and produces detailed graphical reports. In FlowJo, samples are organized in a "Workspace" window, which presents a hierarchical view of all the samples and their analyses (gates and statistics). FlowJo will import and analyze cytometry data regardless of which flow cytometer is used to collect the data. Files produced by modern flow cytometers are written in the Flow Cytometry Standard format with an. JSTOR ( October 2009) ( Learn how and when to remove this template message)įlowJo is a software package for analyzing flow cytometry data.Please improve this article by adding secondary or tertiary sources. Notepadâ¢, MSWordâ¢, TextWranglerâ¢) and save the file.This article relies excessively on references to primary sources. To save the node specific XML, copy and paste the contents in the window to a text editor (e.g.Select the âShow XML for selected nodesâ option from the drop down menu.Select a population(s), statistic(s), or platform specific node(s) in the workspace by clicking on it once.Opening and Saving node/population specific XML. Notepad, MSWord, TextWrangler) and save the file. To save the workspace XML, copy and paste the contents in the window to a text editor (e.g.A new window will open with XML script.Select the âShow XML from Workspaceâ option.Click the Power Band and choose the âShow Diagnosticâ tool from the menu.For information on how to add/remove ribbons, please see this page. Ensure that the Power Band is in the ribbon.This section will show you how to view Workspace XML or Node/Population specific XML. For example, you may have a gating path that includes Singlets, Live, Lymphocytes, CD3+, and CD8+ nodes (or populations). You can also view XML specific to a particular node within a workspace. The Workspace XML option reveals all of a workspacesâ entities (files, populations, gates, fonts, tables, layouts etc.). You can view Workspace or node/population XML using the tools contained in the Power Band. Programs other than FlowJo would not necessarily interpret this XML code. In the above example this would be <â or after the â</â are generally program specific marks, and thus only the program that writes those marks can use them. Other items arenât as clear âeventsInside=1â and annoOffsetX=0 but we can still deduce this is information about a gate. We can gather that this is information about some type of gate on the FSC-A parameter, probably is not tinted, and uses a normal line weight. Consider the following XML line from a FlowJo vX workspace: Most text elements contained within greater than/less than brackets () can be interpreted by the casual user. It is intended to be both machine and human readable to a certain extent. XML is a so-called markup language similar to HTML (the language of the web). However, FlowJo v10 is and can be, used in a 21 CFR part 11 compliant manner. *Note: FlowJo v10 currently does not have a locking mechanism to authorize a user, or to prevent unauthorized modification of a workspace. In addition, workspace XML can be fed into other downstream programs for fitting flow cytometry data or results into a data analysis pipeline. In conjunction with activity logs, it is possible to compare modifications to a workspace between saves, and by extension, between users*. Workspace XML is useful for comparison of a common workspace or between separate workspaces. FlowJo v10 also provides a shortcut to view XML at the node or population level and is described in the Opening and Saving Workspace XML section below. That is, gate dimensions, positions of vertices, file and node names, gating trees, statistics, layouts, plates, tables and formulas are all recorded for each FlowJo v10 workspace. You can view a workspaceâs XML to reveal all that is contained within it. An entire FlowJo v10 workspace is described by XML. ![]()
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